کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815795 1159893 2015 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Integrated analysis of gene expression and microRNA regulation in three leukemia-related lymphoblastic cell lines
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Integrated analysis of gene expression and microRNA regulation in three leukemia-related lymphoblastic cell lines
چکیده انگلیسی


• We sequenced three lymphoblastic cell lines both of mRNA and miRNA expression.
• Characteristics of pre-B lymphoblastic leukemia cell and T lymphoblastic cell were decoded by DEGseq and KEGG annotation.
• Key oncogenes and miRNAs are involved in the network regulation of leukemogenesis and lymphocyte differentiation.
• Leukemia-related miRNAs could give insight into the biological study of diseases.

RNA-sequencing technology is progressively being applied in various fields since high-throughput data analysis provides deeper mining on both the genomic and transcriptomic level. Lymphoblastic leukemogenesis is a complex process caused by abnormalities occurring during lymphocyte differentiation, and can be initiated by various triggers. Each leukemia subtype has distinct characteristics that can be identified in the corresponding cell lines; the detection of the exclusive genetic signatures of these varying cell lines is critical.Our analysis revealed that approximately 8000 human genes were differentially expressed between samples. Signaling pathways such as the NOD-like signaling pathway, cell surface receptor signaling pathways, and leukemia-related pathways were significantly regulated, as determined by KEGG annotation. Furthermore, several oncogenes and differentiation-related genes were differentially expressed between leukemia cell lines and lymphocyte types, respectively. Our miRNA analysis demonstrated that the expression of approximately one-third of all expressed miRNAs appeared to be significantly different between the leukemia cell lines studied. We also analyzed the mRNA–miRNA regulatory networks of both lymphocyte differentiation and leukemogenesis to determine key regulators of interest. We combined the results of the mRNA and miRNA analyses in order to investigate the regulatory relationship between them. This study not only identifies differences in the pathways and networks of acute lymphocytic leukemia (ALL) relative to normal lymphocytes, but also identifies unique functional characteristics of lymphoid cells and distinct gene expression patterns during lymphoid development. The discovery of leukemia-related miRNAs may provide meaningful insights into the biology of the disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 564, Issue 1, 10 June 2015, Pages 39–52
نویسندگان
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