کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815809 1159894 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pre- and post-natal muscle microRNA expression profiles of two pig breeds differing in muscularity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Pre- and post-natal muscle microRNA expression profiles of two pig breeds differing in muscularity
چکیده انگلیسی


• Spatial and temporal differences of miRNA expression in pigs relate to muscle traits.
• miRNA-mediated gene regulation contributes to muscle development in pigs.
• Differentially expressed miRNAs target genes of skeletal and muscular systems.

miRNAs regulate the expression of target genes in diverse cellular processes and hence play important roles in physiological processes including developmental timing, patterning, embryogenesis, organogenesis, cell lineage, myogenesis and growth control. A comparative expression analysis of miRNAs expressed in the longissimus dorsi muscle at two prenatal stages (63 and 91 days post-conception (dpc)), and one adult stage (180 days post-natum) in both German Landrace (DL) and Pietrain (Pi) pig breeds was performed using a custom-designed array. During the prenatal stages, miR-199 and the miR-17 families were significantly up-regulated at 63 dpc, whereas miR-1 and miR-133a were overexpressed at 91 dpc. The abundance of several miRNAs was increased in the adult stage compared to 91 dpc including miR-1, miR-133, miR-22(a/b) and miR-29a. Some miRNAs were breed-specific, such as miR-199 and the miR-17 families which were all up-regulated in Pi pigs, while miR-133, miR-181 and miR-214 were up-regulated in DL pigs. Several pathways related to muscle development were enriched with predicted targets for the differentially expressed miRNAs. The dynamic expression and breed-associated regulation of porcine muscle miRNAs suggests a functional role for miRNA-mediated gene regulation during muscle development and phenotypic variations of muscle traits.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 561, Issue 2, 1 May 2015, Pages 190–198
نویسندگان
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