کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2816028 1159911 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning and functional characterization of β-1, 3-glucanase gene from Podophyllum hexandrum — A high altitude Himalayan plant
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Cloning and functional characterization of β-1, 3-glucanase gene from Podophyllum hexandrum — A high altitude Himalayan plant
چکیده انگلیسی


• A seed specific ß-1,3-glucanase from a cold desert plant Podophyllum was cloned.
• Ph-glucanase is with 852 bp and encodes a 31 kDa protein with pI of 4.39.
• Ph-glucanase expression improved seed germination in Arabidopsis under stress.

Podophyllum hexandrum is a high-altitude medicinal plant exploited for its etoposides which are potential anticancer compounds. ß-1, 3-glucanase cDNA was cloned from the germinating seeds of Podophyllum (Ph-glucanase). Glucanases belong to pathogenesis related glycohydralase family of proteins, which also play an important role in endosperm weakening and testa rupture during seed germination. Analysis of cloned nucleotide sequence revealed Ph-glucanase with an open reading frame of 852 bp encoding a protein of 283 amino acids with a molecular mass of 31 kDa and pI of 4.39. In-silico structure prediction of Ph-glucanase showed homology with that of Hevea brasiliensis (3em5B). Structural stability and enhanced catalytic efficiency in harsh climatic conditions possibly due to the presence of glycosyl hydrolase motif (LGIVISESGWPSAG) and a connecting loop towards inner side and well exposed carbohydrate metabolism domain-COG5309, can readily hydrolyse cell wall sugar moieties. Seeds from the transgenic Arabidopsis plants over-expressing Ph-glucanase showed better germination performance against a wide range of temperatures and abscisic acid (ABA) stress. This can be attributed to the accumulation of Ph-glucanase at both transcript and protein levels during the seed germination in transgenic Arabidopsis. Results confirm that the cloned novel seed specific glucanase from a cold desert plant Podophyllum could be used for the manipulation of different plant species seeds against various harsh conditions.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 554, Issue 1, 1 January 2015, Pages 25–31
نویسندگان
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