کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2816413 1159932 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression differences of miRNAs and genes on NF-κB pathway between the healthy and the mastitis Chinese Holstein cows
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Expression differences of miRNAs and genes on NF-κB pathway between the healthy and the mastitis Chinese Holstein cows
چکیده انگلیسی


• Variation of miRNAs and genes on NF-κB pathway between healthy and mastitis cows.
• The total miRNAs decline and most of the genes expression rise in mastitis cows.
• NF-κB pathway is active in mastitis as a result of the decrease of miRNAs.
• NF-κB pathway is inactive as the miRNAs enhancing inhibition in healthy cows.
• Some miRNAs could be used as new markers for the dairy mastitis prognosing.

In order to discover the variation of microRNAs and genes associated with NF-κB signaling pathway between the healthy and the mastitis Chinese Holstein cows, Illumina Deep Sequencing and qRT-PCR are applied to detect 25 kinds of miRNAs (miR-16, miR-125b, miR-15, miR-29a, miR-23b, miR-146, miR-301a, miR-181b, let-7, miR-30b, miR-21, miR-223, miR-27b, miR-10a, miR-143, etc.) expression levels in blood samples and 14 genes (RelA, RelB, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBε, IκBζ, Bcl-3, IKKα, IKKβ, IKKγ/NEMO) relative expression levels in nine tissues. The total number of miRNAs is declining, and RelA, Rel, p105, p100, IκBα, IκBβ, IκBδ, IκBζ, Bcl-3, and IKKα expressions are rising in mastitis individuals. So, we suppose that NF-κB pathway is active in mastitis individuals as a result of the decrease inhibition of miRNAs. While in healthy ones, the NF-κB pathway is inactive, because of the miRNAs enhanced inhibition action. However, the specific regulatory mechanism of miRNAs on NF-κB pathway in mastitis Holstein cows needs further investigation. Moreover, due to obvious expression differences, some miRNAs, especially miR-16 and miR-223, may be used as new markers for the dairy mastitis prognosing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 545, Issue 1, 15 July 2014, Pages 117–125
نویسندگان
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