کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2816559 | 1159941 | 2014 | 7 صفحه PDF | دانلود رایگان |
• We identified NIC6, a protein of unknown function.
• NIC6 helps yeast cells to tolerate toxic levels of nickel concentration.
• NIC6 localizes in multivesicular structures in tobacco epidermal cells.
Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.
Journal: Gene - Volume 538, Issue 2, 1 April 2014, Pages 251–257