کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2816796 1159952 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genome-wide analysis of endosperm-specific genes in rice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Genome-wide analysis of endosperm-specific genes in rice
چکیده انگلیسی


• Genome-wide analysis identified 151 OSEnS genes in rice.
• Functional analysis found that 151 OSEnS genes could be classified into 12 groups.
• The RY repeat was over-represented in promoter regions of OSEnS genes.
• The expression patterns of 13 OSEnS genes were confirmed by qRT-PCR analysis.

The endosperm of the cereal crop is an important nutrient source for humans. It also acts as a critical integrator of plant seed growth and development. Despite its importance, the comprehensive understanding in regulating of endosperm development in rice remains elusive. Here, we performed a genomic survey comprising the identification and functional characterization of the endosperm-specific genes (OsEnS) in rice using Affymetrix microarray data and Gene Ontology (GO) analysis. A total of 151 endosperm-specific genes were identified, and the expression patterns of 13 selected genes were confirmed by qRT-PCR analysis. Promoter regions of the endosperm-specific expression genes were analyzed by PLACE Signal Scan Search. The results indicated that some motifs were involved in endosperm-specific expression regulation, and some cis-elements were responsible for hormone regulation. The bootstrap analysis indicated that the RY repeat (CATGCA box) was over-represented in promoter regions of endosperm-specific expression genes. GO analysis indicated that these genes could be classified into 12 groups, namely, transcription factor, stress/defense, seed storage protein (SSP), carbohydrate and energy metabolism, seed maturation, protein metabolism, lipid metabolism, transport, cell wall related, hormone related, signal transduction, and one unclassified group. Taken together, our results provide informative clues for further functional characterization of the endosperm-specific genes, which facilitate the understanding of the molecular mechanism in rice endosperm development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 530, Issue 2, 10 November 2013, Pages 236–247
نویسندگان
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