Seamless stitching of biosynthetic gene cluster containing type I polyketide synthases using Red/ET mediated recombination for construction of stably co-existing plasmids
Keywords: PKS, polyketide synthase; PCR, polymerase chain reaction; BAC, bacterial artificial chromosomes; LB, Luria-Bertani; Ap, ampicillin; Am, apramycin; Km, kanamycin; Tc, tetracycline; Cm, chloramphenicol; DMSO, dimethyl sulfoxideto; IPTG, isopropyl-β-d-thioga