کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2817379 | 1159985 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer](/preview/png/2817379.png)
The epigenetic regulation of microRNAs is one of several mechanisms underlying carcinogenesis. We found that microRNA-195 (miR-195) and microRNA-378 (miR-378) were significantly down-regulated in gastric cancer tissues and gastric cancer cell lines. The expression of miR-195 and miR-378 in gastric cancer cells was significantly restored by 5-aza-dC, a demethylation reagent. The low expression of miR-195 and miR-378 was closely related to the presence of promoter CpG island methylation. Treatment with miR-195/miR-378 mimics strikingly suppressed the growth of gastric cancer cells whereas promoted the growth of normal gastric epithelial cells. In contrast, administration of miR-195/miR-378 inhibitors significantly prevented the growth of normal gastric epithelial cells. Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378, respectively. In conclusion, miR-195 and miR-378 are abnormally expressed and epigenetically regulated in gastric cancer cell lines and tissues via the suppression of CDK6 and VEGF signaling, suggesting that miR-195 and miR-378 have tumor suppressor properties in gastric cancer.
► The epigenetic regulation of microRNAs is one of the mechanisms of carcinogenesis.
► MicroRNA-195/378 was significantly down-regulated in gastric cancer cells.
► The growth of gastric cancer cells was suppressed by microRNA-195/378 mimics.
► MicroRNA-195/378 plays growth inhibitory roles in gastric cancer.
Journal: Gene - Volume 518, Issue 2, 15 April 2013, Pages 351–359