کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2817575 1569849 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a new clock-related element EL-box involved in circadian regulation by BMAL1/CLOCK and HES1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Identification of a new clock-related element EL-box involved in circadian regulation by BMAL1/CLOCK and HES1
چکیده انگلیسی

Several cis-acting elements play critical roles in maintaining circadian expression of clock and clock-controlled genes. Using in silico analysis, we identified 10 sequence motifs that are correlated with the circadian phases of gene expression in the cartilage. One of these motifs, an E-box-like clock-related element (EL-box; GGCACGAGGC), can mediate BMAL1/CLOCK-induced transcription, which is typically regulated through an E-box or E′-box. Expression of EL-box-containing genes, including Ank, Dbp, and Nr1d1 (Rev-erbα), was induced by BMAL1/CLOCK or BMAL1/NPAS2. Compared with the E-box, the EL-box elements had distinct responsiveness to DEC1, DEC2, and HES1: suppressive actions of DEC1 and DEC2 on the EL-box were less potent than those on the E-box. HES1, which is known to bind to the N-box (CACNAG), suppressed enhancer activity of the EL-box, but not the E-box. In the Dbp promoter, an EL-box worked cooperatively with a noncanonical (NC) E-box to mediate BMAL1/CLOCK actions. These findings suggest that in addition to known clock elements, the EL-box element may contribute to circadian regulation of clock and clock-controlled genes.


► We identified a new element, EL-box, from circadian oscillating genes in cartilage.
► EL-box can mediate circadian expression by BMAL1/CLOCK, the master in clock system.
► EL-box is similar to E-box, but has distinct functions in transcription regulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 510, Issue 2, 1 December 2012, Pages 118–125
نویسندگان
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