Identification of differentially expressed genes in response to mercury I and II stress in Trichoderma harzianum

Filamentous fungi are very promising organisms in both the control and the reduction of the amount of heavy metal released by human and industrial activities. In particular, Trichoderma harzianum demonstrated to be tolerant towards different heavy metals, such as mercury and cadmium, even though the mechanism underlying this tolerance is not fully understood. By using a particular strategy of the suppression subtractive hybridization technique, we were able to identify in the strain IMI 393899 of T. harzianum eight different genes up-regulated in the presence of mercury II with respect to cadmium. Among the genes identified, a possible role in the tolerance mechanism could be envisaged for hydrophobin, due to its ability to dissolve hydrophobic molecules into aqueous media. We also show that IMI 393899 grows at the same rate of control culture in the presence of mercury I and that all eight genes isolated were also up-regulated in this condition.

► T. harzianum strain IMI 393899 is able to grow in the presence of HgCl2, CdSO4 and Hg2Cl2.
► A particular strategy of suppression subtractive hybridization was successfully used.
► The strategy used lead to the isolation of eight differentially expressed genes.