کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2817769 1160011 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biochemical and molecular analyses of copper–zinc superoxide dismutase from a C4 plant Pennisetum glaucum reveals an adaptive role in response to oxidative stress
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Biochemical and molecular analyses of copper–zinc superoxide dismutase from a C4 plant Pennisetum glaucum reveals an adaptive role in response to oxidative stress
چکیده انگلیسی

Superoxide dismutases (SODs) form the foremost line of defense against ROS in aerobes. Pennisetum glaucum cDNA library is constructed to isolate superoxide dismutase cDNA clone (PgCuZnSOD) of 798 bp comprising 5′UTR (111 bp), an ORF (459 bp) and 3′UTR (228 bp). Deduced protein of 152 amino acids (16.7 kDa) with an estimated isoelectric point of 5.76 shared highest homology to cytoplasmic CuZnSODs from monocots i.e., maize, rice. Predicted 3D model reveals a conserved eight-stranded ß-barrel with active site held between barrel and two surface loops. Purified recombinant protein is relatively thermo-stable with maximal activity at pH 7.6 and shows inhibition with H2O2 (4.3 mM) but not with azide (10 mM). In Pennisetum seedlings, abiotic stress induced PgCuZnSOD transcript up-regulation directly correlates to high protein and activity induction. Overexpression of PgCuZnSOD confers comparatively enhanced tolerance to methyl viologen (MV) induced oxidative stress in bacteria. Results imply that PgCuZnSOD plays a functional role in conferring oxidative stress tolerance to prokaryotic system and may hold significant potential to impart oxidative stress tolerance in higher plants through transgenic approach.


► We analyzed the protective role of PgCuZnSOD in response to oxidative stress.
► Transcript upregulation directly correlates to high protein and activity induction.
► PgCuZnSOD confers oxidative stress tolerance to prokaryotic system.
► PgCuZnSOD may also impart oxidative stress tolerance to higher plants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 505, Issue 2, 1 September 2012, Pages 309–317
نویسندگان
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