کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2818519 1569866 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reducing chimera formation during PCR amplification to ensure accurate genotyping
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Reducing chimera formation during PCR amplification to ensure accurate genotyping
چکیده انگلیسی

Measurements of population diversity are fundamental to the reconstruction of the evolutionary and epidemiological history of organisms. Commonly used protocols to measure population diversity using the polymerase chain reaction (PCR) are prone to the introduction of artificial chimeras. These are often difficult to detect and can confound the correct interpretation of results due to the false generation of recombinants when the underlying DNA sample contains multiple distinct templates. This study presents a standardised procedure to suppress the formation of artificial chimeras during PCR amplification. The solution is based on the accurate determination of the efficiency and end point of the log-linear phase of a PCR. This procedure will facilitate the generation of data sets that more accurately reflect the underlying population diversity rather than artifacts introduced by the process itself.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 469, Issues 1–2, 1 December 2010, Pages 45–51
نویسندگان
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