کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2818836 1569888 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene
چکیده انگلیسی

Glycine N-methyltransferase (GNMT) is a tumor susceptibility gene for both hepatocellular carcinoma and prostate cancer. We have previously characterized GNMT genomic structure and mapped its chromosomal localization to 6p12. For this study we identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon. Electrophoretic mobility shift assay results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (− 71/− 67) of the GNMT gene. Mutation assay results suggest that the nucleotide sequence in the − 56/− 47 region is a binding site for a putative transcriptional factor. The TATA-less core promoter (− 133/+ 14) contains three major elements: an Sp1 site, CCAAT box, and a novel box within the CTGTCGGCTG sequence. One functional xenobiotic response element (XRE) located at the − 104/− 82 region is inducable by benzo[a]pyrene treatment. We believe our results have value for the study of GNMT transcriptional regulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 443, Issues 1–2, 15 August 2009, Pages 151–157
نویسندگان
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