Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CD, circular dichroism; ChIP, chromatin immunoprecipitation; EMSA, electrophoretic mobility shift assay; FAM, 6-carboxyfluorescein; ITC, isothermal titration calorimetry; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide; NHE III1, nuc
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Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; ANT-1, adenine nucleotide translocator 1; CEE, conjugated equine estrogen; CHD, coronary heart disease; Cx43, Connexin 43; EMSA, electrophoretic mobility shift assay; eNOS, endothelial nitric oxide synthase; ER, estrogen receptor; E2, 17β-estradiol; E2, e
Photoaffinity electrophoretic mobility shift assay using photoreactive DNA bearing 3-trifluoromethyl-3-phenyldiazirine in its phosphate backbone
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Diazirine; Octamer motif; Phosphorothioate; Photo-cross-linking; Proteomic analysisUV, ultraviolet; EMSA, electrophoretic mobility shift assay; SDS–PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; DMSO, dimethyl sulfoxide; HPLC, high-perfo
Andrographolide inhibits adipogenesis of 3T3-L1 cells by suppressing C/EBPβ expression and activation
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CDK, cyclin-dependent kinase; CREB, cAMP response element-binding protein; C/EBP, CCAAT/enhancer-binding protein; EMSA, electrophoretic mobility shift assay; ERK, extracellular signal-regulated kinases; FAS, fatty acid synthase; GSK, glycogen synthase kin
Short-chain C2 ceramide induces heme oxygenase-1 expression by upregulating AMPK and MAPK signaling pathways in rat primary astrocytes
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; C2 ceramide; Astrocyte; Oxidative stress; Phase II antioxidant enzyme; Heme oxygenase-1; Signaling mechanismAMPK, 5′-adenosine monophosphate-activated protein kinase; EMSA, electrophoretic mobility shift assay; ERK, extracellular signal-regulated kinase;
Tetrachlorobenzoquinone exhibits neurotoxicity by inducing inflammatory responses through ROS-mediated IKK/IκB/NF-κB signaling
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; COX-2, cyclooxygenase-2; ELISA, enzyme-linked immunosorbent assay; EMSA, electrophoretic mobility shift assay; ERK1/2, extracellular regulated kinase1/2; HCB, hexachlorobenzene; IκBα, inhibitor of NF-κB; IKK, inhibitor of nuclear factor kappa-B kinase; IL
Functional activity of the porcine Gnrhr2 gene promoter in testis-derived cells is partially conferred by nuclear factor-κB, specificity protein 1 and 3 (SP1/3) and overlapping early growth response 1/SP1/3 binding sites
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; GnRH1, gonadotropin-releasing hormone 1; GnRH2, gonadotropin-releasing hormone 2; GnRHR1, gonadotropin-releasing hormone 1 receptor; GnRHR2, gonadotropin-releasing hormone 2 receptor; ST, swine testis-derived; EMSA, electrophoretic mobility shift assay; N
Phorbol 12-myristate 13-acetate promotes nuclear translocation of hepatic steroid response element binding protein-2
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CVD, cardiovascular disease; RT-PCR, reverse transcriptase-polymerase chain reaction; SCAP, SREBP-cleavage activating protein; COPII, coatamer protein II; S1P, Site-1 protease; S2P, Site-2 protease; EMSA, electrophoretic mobility shift assay; SRE, sterol
Peculiarities of the interaction of the restriction endonuclease BspD6I with DNA containing its recognition site
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; AFM, atomic-force microscopy; Bt, biotin residue; EDTA, ethylenediaminetetraacetic acid; EMSA, electrophoretic mobility shift assay; IC50, half-maximal inhibitory concentration; Kd(app), apparent dissociation constant; MTase, M., methyltransferase; m6A, 6
Early growth response 1 (EGR-1) is a transcriptional regulator of mitochondrial carrier homolog 1 (MTCH 1)/presenilin 1-associated protein (PSAP)
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; MTCH1, mitochondrial carrier homolog 1; PSAP, presenilin 1-associated protein; EGR-1, early growth response 1; EMSA, electrophoretic mobility shift assay; RNAi, RNA interference; siRNA, small interfering RNAMitochondria; Cell death; Apoptosis; Mitochondri
A Selective Small Molecule DNA2 Inhibitor for Sensitization of Human Cancer Cells to Chemotherapy
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; BLM, Bloom Syndrome; CPT, camptothecin; DNA2, DNA2 nuclease/helicase; DSB, double-strand break; EMSA, electrophoretic mobility shift assay; EXO1, exonuclesae 1; FA, Fanconi anemia; FEN1, flap endonuclease 1; HDR, homology direct repair; HTS, high throughp
LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Bombyx mori; Silk gland; Fibroin; Awh; TransgenicASG, anterior silk gland; Awh, Arrowhead; EMSA, electrophoretic mobility shift assay; FMBP-1, fibroin-modulator-binding protein-1; Lcaf, LIM-homeodomain and Ldb complex-associated factor; Ldb, LIM domain-bi
Uric acid promotes chemokine and adhesion molecule production in vascular endothelium via nuclear factor-kappa B signaling
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Uric acid; Hyperuricemia; Atherosclerosis; Chemokines; Adhesion molecules; NF-κBUA, uric acid; HUVECs, human umbilical vein endothelial cells; URAT1, urate transporter-1; NF-κB, nuclear factor-kappa B; EMSA, electrophoretic mobility shift assay; eNOS, end
c-ETS transcription factors play an essential role in the licensing of human MCM4 origin of replication
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; BrdU, 5-bromo-2′-deoxy-uridine; ChiP, chromatin immunoprecipitation; DMSO, dimethylsulfoxide; DNMT, DNA methyltransferase; EMSA, electrophoretic mobility shift assay; HAT, histone acetyltransferase; HDAC, histone deacetylase; MCM4, minichromosome maintena
Activation of NF-κB signaling pathway in HSV-1-induced mouse facial palsy: Possible relation to therapeutic effect of glucocorticoids
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; COX-2, cyclooxygenase-2; DIG, digoxigenin; EDTA, ethylenediaminetetraacetic acid; ELISA, enzyme-linked immunosorbent assay; EMSA, electrophoretic mobility shift assay; GCs, Glucocorticoids; GR, glucocorticoid receptor; HEPES, 4-(2-hydroxyethyl)-1-piperazi
Lysine Deacetylases Regulate the Heat Shock Response Including the Age-Associated Impairment of HSF1
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; HSF1, heat shock factor 1; HSR, heat shock response; HSE, heat shock element; EMSA, electrophoretic mobility shift assay; DBD, DNA binding domain; RD, regulatory domain; AD, activation domain; MEF, mouse embryonic fibroblast; PD, population doubling; GR,
Macrophage migration inhibitory factor promotes expression of GLUT4 glucose transporter through MEF2 and Zac1 in cardiomyocytes
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; MIF, macrophage migration inhibitory factor; GLUT4, facilitated glucose transporter, member 4; DCM, diabetic cardiomyopathy; RNAi, RNA interference; EMSA, electrophoretic mobility shift assay; NMVCs, neonatal mouse ventricular cardiomyocytes; T1D, type 1
Polydatin promotes Nrf2-ARE anti-oxidative pathway through activating Sirt1 to resist AGEs-induced upregulation of fibronetin and transforming growth factor-β1 in rat glomerular messangial cells
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; AGEs, advanced glycation-end products; ARE, anti-oxidant response element; BSA, bovine serum albumin; DN, diabetic nephropathy; ECM, extracellular matrix; EMSA, electrophoretic mobility shift assay; FN, fibronectin; GMCs, glomerular mesangial cells; HG, h
Characterization of the termini of the West Nile virus genome and their interactions with the small isoform of the 2′ 5′-oligoadenylate synthetase family
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CS, conserved sequence; DLS, dynamic light scattering; Dmax, maximum particle dimension; dsRNA, double-stranded RNA; EMSA, electrophoretic mobility shift assay; IFN, interferon; ISG, interferon-stimulated genes; ITC, isothermal titration calorimetry; NSD,
Conserved transcription factor binding sites suggest an activator basal promoter and a distal inhibitor in the galanin gene promoter in mouse ES cells
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Bp, Base pair; ChIP, Chromatin immunoprecipitation; DR, Distal region; EDTA, Ethylenediaminetetraacetic acid; EMSA, Electrophoretic mobility shift assay; ES, Embryonic stem cells; HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; kb, Kilobase; KC
Sp1 transcriptionally regulates BRK1 expression in non-small cell lung cancer cells
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; NSCLC, non-small cell lung cancer; EMSA, electrophoretic mobility shift assay; ChIP, chromatin immunoprecipitation; IHC, immunohistochemistry; RLU, relative luciferase units; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; PVDF, polyv
Structural and genomic DNA analysis of the putative TetR transcriptional repressor SCO7518 from Streptomyces coelicolor A3(2)
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; HTH, helix-turn-helix; PCR, polymerase chain reaction; gSELEX, genomic systematic evolution of ligands by exponential enrichment; FITC, fluorescein isothiocyanate; EMSA, electrophoretic mobility shift assay; IR, inverted repeat; N-DBD, N-terminal DNA-bind
Molecular mechanisms of herbicide-inducible gene expression of tobacco CYP71AH11 metabolizing the herbicide chlorotoluron
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CYP, cytochrome P450; 2,4-D, (2,4-dichlorophenoxy) acetic acid; EMSA, electrophoretic mobility shift assay; GUS, β-glucuronidase; ROS, reactive oxygen speciesBromoxynil; Chlorotoluron; Cytochrome P450 71AH11; Reactive oxygen species
Excisions of a defective transposable CACTA element (Tetu1) generate new alleles of a CYCLOIDEA-like gene of Helianthus annuus
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; bp, base pair(s); BiFC, Bimolecular Fluorescence Complementation; Chry2, Chrysanthemoides2; CMT3, CHROMOMETHYLASE3; CYC, CYCLOIDEA; DDM1, DNA METHYLATION1; EMSA, Electrophoretic Mobility Shift Assay; En/Spm, Enhancer/Suppressor–mutator; MET1, METHYLTRANSF
Wogonin inhibits H2O2-induced angiogenesis via suppressing PI3K/Akt/NF-κB signaling pathway
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; ROS, reactive oxygen species; NF-κB, nuclear factor-κB; IκB, inhibitor-κB; IKK, IκB kinase; VEGF, vascular endothelial growth factor; ELISA, enzyme linked immunosorbent assay; EMSA, electrophoretic mobility shift assay; CAM, chicken chorioallantoic membra
Downregulation of connective tissue growth factor by LPS/IFN-γ-induced nitric oxide is reversed by aristolochic acid treatment in glomerular mesangial cells via STAT-1α and NF-κB signaling
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; AA, aristolochic acid; AAN, AA nephropathy; CTGF, connective tissue growth factor; CHN, chinese herb nephropathy; CKD, chronic kidney disease; CM, cytokine mixtures; ECM, extracellular matrix; EMSA, electrophoretic mobility shift assay; GAS, gamma-activat
Identification of two novel functional p53 responsive elements in the herpes simplex virus-1 genome
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; 5-FU, 5-fluorouracil; ChIP, chromatin immunoprecipitation; EMSA, electrophoretic mobility shift assay; FBS, fetal bovine serum; HSV, herpes simplex virus; IE, immediate early; moi, multiplicity of infection; PBS, phosphate buffered saline; RE, responsive
The Transcriptional Repressor ZBTB4 Regulates EZH2 Through a MicroRNA-ZBTB4-Specificity Protein Signaling Axis 12
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Sp, Specificity protein; ZBTB4, Zinc finger BTB domain protein 4; miR, microRNA; BA, Betulinic acid; EMSA, Electrophoretic mobility shift assay; Chip, Chromatin immunoprecipitation assay
The bidirectional p53–Wrap53β promoter is controlled by common cis- and trans-regulatory elements
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; bp, base pairs; E. coli, Escherichia coli; EDTA, ethylenediaminetetraacetic acid; EMSA, electrophoretic mobility shift assay; FBS, fetal bovine serum; h, hour; HSV, herpes simplex virus; Nspc, non-specific competitor; Nuc, nucleotide; S phase, synthesis p
An Arabidopsis R2R3-MYB transcription factor, AtMYB20, negatively regulates type 2C serine/threonine protein phosphatases to enhance salt tolerance
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; ABA, abscisic acid; AtMYB20, Arabidopsis thaliana MYB20; EMSA, electrophoretic mobility shift assay; GUS, β-glucuronidase; MS medium, Murashige and Skoog medium; PP2Cs, type 2C serine/threonine protein phosphatases; RT-PCR, reverse transcription-PCR; TF,
Interplay between KLF4 and ZEB2/SIP1 in the regulation of E-cadherin expression
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; EMT, epithelial-to-mesenchymal transition; ChIP, chromatin immunoprecipitation; EMSA, electrophoretic mobility shift assay; DOX, doxycyclineKLF4; ZEB2; E-cadherin; EMT; Breast cancer; Transcriptional regulation
Regulation of CEP131 gene expression by SP1
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; EMSA, electrophoretic mobility shift assay; ChIP, chromatin immuno-precipitation; CEP131, centrosomal protein of 131 kDa; SP1, specificity protein 1; DHFR, dihydrofolate reductase; PARP-1, poly (ADP-ribose) polymerase-1; CPAP, centrosomal P4.1-associated
Protopanaxatriol ginsenoside Rh1 inhibits the expression of matrix metalloproteinases and the in vitro invasion/migration of human astroglioma cells
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; AP, activator protein; EMSA, electrophoretic mobility shift assay; ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; MAPK, mitogen-activated protein kinase; MMP, matrix metalloproteinase; NF-κB, nuclear factor-κB; PI3K, phosphoinos
Potential antipsoriatic effect of chondroitin sulfate through inhibition of NF-κB and STAT3 in human keratinocytes
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CS, chondroitin sulfate; EMSA, electrophoretic mobility shift assay; NHK, normal human keratinocyte; OA, osteoarthritis; STAT, signal transducers and activators of transcription; TPA, 12-O-tetradecanoylphorbol 13-acetate; CCL27/CTACK, cutaneous T cell-att
Role of hypoxia inducible factor-1α in the regulation of the cancer-specific variant of organic anion transporting polypeptide 1B3 (OATP1B3), in colon and pancreatic cancer
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; OATP1B3; Splicing variants; Hypoxia; Colon cancer; Pancreatic cancerOATP, organic anion transporting polypeptide; WT, wildtype; HRE, hypoxia response element; HIF-1, hypoxia inducible factor-1; EMSA, electrophoretic mobility shift assay; RT-PCR, reverse t
The TGFβ1 pathway is required for NFκB dependent gene expression in mouse keratinocytes
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; EMSA, electrophoretic mobility shift assay; TGFβ1, transforming growth factor-beta 1; TNFα, tumor necrosis factor alpha; NFkappaB, nuclear factor kappaB; IKβ, I kappaβ; IKβsr, I kappaβ super repressor; TAK1, TGFβ1 activated kinase 1; IKKα, I kappa Kinaseα
Synthesis, characterization and in vitro activity of thrombin-binding DNA aptamers with triazole internucleotide linkages
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Anticoagulants; Thrombin inhibition; DNA aptamers; G-Quadruplexes; Triazole internucleotide linkagesCD, circular dichroism; EDTA, ethylendiamine tetraacetic acid; EMSA, electrophoretic mobility shift assay; HPLC, high performance liquid chromatography; HR
Regulation of N-Myc downstream regulated gene 2 by bile acids
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CDCA, chenodeoxycholic acid; EMSA, electrophoretic mobility shift assay; ENaC, epithelial sodium channel; FXR, farnesoid X receptor; HCC, hepatocellular carcinoma; I-BABP, ileal-bile acid-binding protein; IR1, inverted repeat separated by a nucleotide; MO
A distal estrogen responsive element upstream the cap site of human transthyretin gene is an enhancer-like element upon ERα and/or ERβ transactivation
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; E2, 17 beta-estradiol; B-gal, beta-galactosidase; CSF, cerebrospinal fluid; CP, choroid plexus; EREcon, consensus oligonucleotide for ERE; EMSA, electrophoretic mobility shift assay; pGL2-P/TTR, ERETTR cloned in pGL2 vector; ER, estrogen receptor; ERα, es
H. pylori-encoded CagA disrupts tight junctions and induces invasiveness of AGS gastric carcinoma cells via Cdx2-dependent targeting of Claudin-2
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CagA, cytotoxin-associated gene A; FCS, fetal calf serum; EDTA, ethylenediaminetetraacetic acid disodium salt; AJC, apical junctional complex; EMSA, electrophoretic mobility shift assay; ZO-1, zonulae occludentes-1; HNF, hepatocyte nuclear factor; DS, des
The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; HBXIP, hepatitis B X-interacting protein; Skp2, S-phase kinase-associated protein 2; ChIP, chromatin immunoprecipitation; Co-IP, co-immunoprecipitation; EMSA, electrophoretic mobility shift assay; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
Piperlongumine inhibits proliferation and survival of Burkitt lymphoma in vitro
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Ab(s), Antibody(ies); BL, Burkitt lymphoma; CE, Cytoplasmic protein extract; E2F1, E2F transcription factor 1; EBV, Epstein Barr virus; EMSA, Electrophoretic mobility shift assay; GADD45B, Growth arrest and DNA-damage-inducible 45 beta; LMP-1, Latent memb
Ethyl pyruvate ameliorates intracerebral hemorrhage-induced brain injury through anti-cell death and anti-inflammatory mechanisms
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; EDTA, ethylenediamine tetraacetic acid; ELISA, enzyme-linked immunosorbent assay; EMSA, electrophoretic mobility shift assay; EP, ethyl pyruvate; FJC, Fluoro-Jade C; ICH, intracerebral hemorrhage; IL-1β, interleukin-1β; MTT, 3-(4,5-dimethylthiazol-2-yl)-2
14-3-3ζ interacts with hepatocyte nuclear factor 1α and enhances its DNA binding and transcriptional activation
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; HNF, hepatocyte nuclear factor; MODY, maturity onset diabetes of the young; OA, okadaic acid; CIP, calf intestinal alkaline phosphatase; 14-3-3ζ, 14-3-3zeta; ChIP, chromatin-immunoprecipitation; EMSA, electrophoretic mobility shift assay; FRET, fluorescen
A thermodynamic model of the cooperative interaction between the archaeal transcription factor Ss-LrpB and its tripartite operator DNA
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Bp, base pair; BRE, factor B recognition element; Cu-OP, copper-phenantroline; EMSA, electrophoretic mobility shift assay; FL, full-length; HTH, helix-turn-helix; KBi, equilibrium association constant for binding at box Bi; Ki, stepwise equilibrium consta
Hepatitis E: Molecular Virology and Pathogenesis
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; Hepeviridae; open reading frames; pathogenesisRdRp, RNA dependent RNA polymerase; ORF, open reading frames; UTR, untranslated region; NTP, nucleoside triphosphate; ER, endoplasmic reticulum; EMSA, electrophoretic mobility shift assay; ELISPOT, enzyme-link
A recombinase paralog from the hyperthermophilic crenarchaeon Sulfolobus solfataricus enhances SsoRadA ssDNA binding and strand displacement
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; dsDNA, double-strand DNA; ssDNA, single-strand DNA; Gy, Gray; IR, ionizing radiation; UV, ultraviolet; DSB, double-strand break; HR, homologous recombination; bp, basepairs; EMSA, electrophoretic mobility shift assay; ORF, open reading frameArchaea; Recom
Tools and strategies for DNA damage interactome analysis
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; DDR, DNA damage response; DIGE, differential in-gel electrophoresis; DPC, DNA-protein crosslink; EMSA, electrophoretic mobility shift assay; ESI-MS/MS, electrospray ionisation tandem mass spectrometry; FRET, fluorescence resonance energy transfer; IRIF, i
On the Origin of the Selectivity of Plasmidic H-NS towards Horizontally Acquired DNA: Linking H-NS Oligomerization and Cooperative DNA Binding
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; DBD, DNA-binding domain; dsDNA, double-stranded DNA; EDTA, ethylenediaminetetraacetic acid; EMSA, electrophoretic mobility shift assay; HGT, horizontal gene transfer; HSQC, heteronuclear single quantum coherenceallosteric interactions; indirect DNA readou
The Non-Coding B2 RNA Binds to the DNA Cleft and Active-Site Region of RNA Polymerase II
Keywords: EMSA، روش تحرک حرکت الکتروفورتیک; CTD, C-terminal domain of RPB1; EMSA, electrophoretic mobility shift assay; MS, mass spectrometry; ncRNA, non-coding RNA; Pol II, RNA polymerase II; RPB, subunit of RNA polymerase II; TBP, TATA binding protein; TFII, transcription factor of RNA polymerase