Structural and genomic DNA analysis of the putative TetR transcriptional repressor SCO7518 from Streptomyces coelicolor A3(2)

• The crystal structure of SCO7518, a putative transcriptional regulator, was determined.
• gSELEX and EMSA analyses identified the operator sequence of SCO7518.
• SCO7518 recognizes an operator sequence located upstream of the sco7519 gene.

SCO7518 is a protein of unknown function from Streptomyces coelicolor A3(2) that has been classified into the TetR transcriptional regulator family. In this study, a crystal structure of SCO7518 was determined at 2.29 Å resolution. The structure is a homodimer of protomers that comprise an N-terminal DNA-binding domain and a C-terminal dimerization and regulatory domain, and possess a putative ligand-binding cavity. Genomic systematic evolution of ligands by exponential enrichment and electrophoretic mobility shift assays revealed that SCO7518 specifically binds to an operator sequence located upstream of the sco7519 gene, which encodes a maltose O-acetyltransferase. These results suggest that SCO7518 is a transcriptional repressor of sco7519 expression.

Structured summary of protein interactionsSCO7518 and SCO7518bind by x-ray crystallography (View interaction)