کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2818985 1569895 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Abundant expression of Kallikrein 1 gene in human keratinocytes was mediated by GATA3
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Abundant expression of Kallikrein 1 gene in human keratinocytes was mediated by GATA3
چکیده انگلیسی

Among Tissue kallikrein genes (KLKs), KLK1 is abundantly expressed in human skin. Although its putative promoter is known to have various cis-elements, they have not been functionally tested. In the present study, the regulation mechanism of KLK1 promoter supporting such abundant expression was examined. Luciferase assay targeting the KLK1 promoter (nucleotide − 1153/+ 40 from the major transcriptional start site) was performed on NHEK human keratinocyte. − 954/− 855, − 428/− 236, and − 100/+ 40 had the induction activity. The motif search program failed to find unique binding motifs in − 428/− 236, whereas both − 954/− 855 and − 100/+ 40 had a unique GATAs binding motif. Electrophoretic mobility shift assay (EMSA) and DNA footprinting confirmed the binding of NHEK nuclear protein to these motifs that were supershifted by anti-GATA3 antibody. Among GATA isoforms, GATA3 alone could be amplified in RNA obtained from NHEK. Moreover, introduction of GATA3 into fibroblastic NIH3T3 cells enhanced the activity of KLK1 promoter containing − 954/+ 40, while that of GATA3 dominant negative mutant to NHEK cells impaired the same promoter's activity. Thus, GATA3 was found to bind the site located at − 954/− 855 and to be a key regulator of abundant KLK1 expression in human keratinocyte.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 436, Issues 1–2, 1 May 2009, Pages 121–127
نویسندگان
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