کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2819239 1569913 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase
چکیده انگلیسی

Patatin-domain containing proteins constitute a large family of enzymes including known lipases that hydrolyze triglycerides, diglycerides, and phospholipids, some of which still remain to be characterized. One of those is NTE-related esterase (NRE), which exhibits sequence and domain homology to neuropathy target esterase (NTE). A splice variant of the catalytic domain of mouse NRE (mNRECV) was identified in multiple adult tissues, including brain, kidney, liver and testis. Genomic organization showed that mNRECV gene lacked the 22nd exon of mouse NRE and the 14th exon termination site of mNRECV was behind of 5 bp with the comparison of the 34th exon of mNRE gene. Over-expression of mNREC and mNRECV in mammalian cells showed that they had similar phenyl valerate esterase activities, but different from human NTE esterase domain. Subcellular distribution of an enhanced green fluorescent protein-mNRECV fusion protein was mainly observed to colocalize with endoplasmic reticulum in the juxtanuclear area and a little in cytoplasm. Moreover, autophagy/lysosome pathway was found to be involved in the degradation of mNRECV protein by inhibition and induction of autophagy, as well as co-location of mNRECV-EGFP with lysosomes. The high identity between mNRECV and mNREC suggested that mouse NRE has similar characteristics.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 417, Issues 1–2, 1 July 2008, Pages 43–50
نویسندگان
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