کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2819476 | 1569923 | 2008 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Myocyte enhancer factor 2 regulates expression of medaka Oryzias latipes fast skeletal myosin heavy chain genes in a temperature-dependent manner
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
We characterized the promoter activity of fast skeletal myosin heavy chain genes (MYHs) from medaka Oryzias latipes. The 5â²-flanking region of â¼Â 6 kb in medaka MYHs, mMYH10 and mMYH30, predominantly expressed in medaka acclimated to 10 °C and 30 °C, respectively, contained various cis-elements that are supposed to bind to transcriptional regulatory factors such as MyoD and myocyte enhancer factor 2 (MEF2) family members and nuclear factor of activated T cells. To localize functional regions responsible for the mMYH expression in a temperature-dependent manner, a series of deletion and site mutation constructs prepared from the 5â²-flanking regions were fused to the luciferase gene in a commercially available plasmid and directly injected into the dorsal fast muscle of medaka acclimated to 10 °C and 30 °C. The truncation of MEF2 binding site located at â 966 to â 957 in the 5â²-flanking region of mMYH10 resulted in distinct gene expression at 10 °C. The activation effect by the removal of this binding site was further confirmed by the mutation construct. One of the E box sites, to which MyoD family members are supposed to bind, was located at â 613 to â 607 of mMYH10, and found to be responsible for the transcriptional activity. In contrast, the MEF2 binding site located at â 960 to â 951 of mMYH30 was involved in the activation at 30 °C. Thus, these transient transfection assays demonstrated that the MEF2 binding site is crucial for a temperature-dependent expression of mMYHs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 407, Issues 1â2, 15 January 2008, Pages 42-53
Journal: Gene - Volume 407, Issues 1â2, 15 January 2008, Pages 42-53
نویسندگان
Chun-Shi Liang, Daisuke Ikeda, Shigeharu Kinoshita, Atsushi Shimizu, Takashi Sasaki, Shuichi Asakawa, Nobuyoshi Shimizu, Shugo Watabe,