کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2819719 | 1569935 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Molecular cloning and characterization of a novel form of the human vacuolar H+-ATPase e-subunit: An essential proton pump component Molecular cloning and characterization of a novel form of the human vacuolar H+-ATPase e-subunit: An essential proton pump component](/preview/png/2819719.png)
Several of the 13 subunits comprising mammalian H+-ATPases have multiple alternative forms, encoded by separate genes and with differing tissue expression patterns. These may play an important role in the intracellular localization and activity of H+-ATPases. Here we report the cloning of a previously uncharacterized human gene, ATP6V0E2, encoding a novel H+-ATPase e-subunit designated e2. We demonstrate that in contrast to the ubiquitously expressed gene encoding the e1 subunit (previously called e), this novel gene is expressed in a more restricted tissue distribution, particularly kidney and brain. We show by complementation studies in a yeast strain deficient for the ortholog of this subunit, that either form of the e-subunit is essential for proper proton pump function. The identification of this novel form of the e-subunit lends further support to the hypothesis that subunit differences may play a key role in the structure, site and function of H+-ATPases within the cell.
Journal: Gene - Volume 393, Issues 1–2, 15 May 2007, Pages 94–100