کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2820270 1569950 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Salmonella-based, propionate-inducible, expression system for Salmonella enterica
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
A Salmonella-based, propionate-inducible, expression system for Salmonella enterica
چکیده انگلیسی

The expression and regulatory properties of a propionate-regulated overexpression system (Salmonella enterica prpBCDE promoter (PprpB) and positive regulator (prpR)) were evaluated in wild-type S. enterica serovar Typhimurium TR6583 and prpB− or prpD− versions of this strain and compared with the arabinose-regulated T7 expression system. The wild-type strain showed low expression in the absence of propionate and high expression in the presence of propionate under all growth conditions. In 96-well plates and culture tubes, the wild-type strain exhibited a long delay before full induction; the time delay was significantly shorter in shake flasks. The prpD− strain exhibited low expression in the presence of glucose, highly regulatable expression over a wide range of propionate concentrations, and, in contrast to the wild-type strain, fast induction to full expression under all growth conditions. In contrast, the prpB− strain showed very high background expression in both culture tubes and shake flasks.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 377, 1 August 2006, Pages 6–11
نویسندگان
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