Characterization, organization and expression of AmphiLysC, an acidic c-type lysozyme gene in amphioxus Branchiostoma belcheri tsingtauense

The study on lysozymes remains open in amphioxus, a cephalochordate. Here we show the existence of c-type lysozyme gene (AmphiLysC) in amphioxus, first such data in the basal chordates including urochordate and cephalochordate. This is in contrast to the absence of c-type lysozyme genes in urochordate. It is found that there exist two copies of c-type lysozyme genes in amphioxus genome, and their gene organization is similar to vertebrate c-type lysozyme genes with respect to the number and the size of both exons and introns. AmphiLysC possesses main features characteristic of the digestive c-type lysozyme such as lower number of basic amino acids (low pI values) and pH-optimum in acidic range. Moreover, AmphiLysC is predominantly expressed in the gut. These indicate that AmphiLysC is possibly a digestive c-type enzyme. However, the ubiquitous expression of AmphiLysC in non-digestive tissues such as ovaries, testes, notochord, gill and muscle suggests that it may also play a non-digestive role like antibacterial activity. It is highly likely that AmphiLysC is an enzyme with a combined function of digestion and bacteriolysis.