کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2822238 1570124 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptomic profiling of primary alveolar epithelial cell differentiation in human and rat
ترجمه فارسی عنوان
پروفیل ترانسکتومومیک تمایز سلول های اپیتلیال آلوئولار اولیه در انسان و موش
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی

Cell-type specific gene regulation is a key to gaining a full understanding of how the distinct phenotypes of differentiated cells are achieved and maintained. Here we examined how changes in transcriptional activation during alveolar epithelial cell (AEC) differentiation determine phenotype. We performed transcriptomic profiling using in vitro differentiation of human and rat primary AEC. This model recapitulates in vitro an in vivo process in which AEC transition from alveolar type 2 (AT2) cells to alveolar type 1 (AT1) cells during normal maintenance and regeneration following lung injury. Here we describe in detail the quality control, preprocessing, and normalization of microarray data presented within the associated study (Marconett et al., 2013). We also include R code for reproducibility of the referenced data and easily accessible processed data tables.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics Data - Volume 2, December 2014, Pages 105–109
نویسندگان
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