کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2822252 1570124 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genome-wide copy number profiling to detect gene amplifications in neural progenitor cells
ترجمه فارسی عنوان
پروفیل تعداد کپی ژنوم برای تشخیص تکثیر ژن در سلول های پیش آگهی دهنده عصبی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی

DNA sequence amplification occurs at defined stages during normal development in amphibians and flies and seems to be restricted in humans to drug-resistant and tumor cells only. We used array-CGH to discover copy number changes including gene amplifications and deletions during differentiation of human neural progenitor cells. Here, we describe cell culture features, DNA extraction, and comparative genomic hybridization (CGH) analysis tailored towards the identification of genomic copy number changes. Further detailed analysis of amplified chromosome regions associated with this experiment, was published by Fischer and colleagues in PLOS One in 2012 (Fischer et al., 2012). We provide detailed information on deleted chromosome regions during differentiation and give an overview on copy number changes during differentiation induction for two representative chromosome regions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics Data - Volume 2, December 2014, Pages 162–165
نویسندگان
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