کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2824117 1570351 2013 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Abundance and diversity of plasmid-associated genes among clinical isolates of Enterococcus faecalis
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Abundance and diversity of plasmid-associated genes among clinical isolates of Enterococcus faecalis
چکیده انگلیسی


• We examined 152 clonally and clinically diverse group of hospital isolates of E. faecalis.
• There is a high content of various plasmid-associated genes in E. faecalis.
• Differences in distribution of particular plasmid genes and their alleles exist between HIRECC and non-HIRECC isolates.
• Pheromone plasmid-associated genes show a significant degree of variability.

Enterococcus faecalis, a normal compound of the human intestinal microbiome, plays an important role in hospital-acquired infections. Plasmids make a significant contribution to the acquisition of the novel traits such as antimicrobial resistance and virulence by this pathogen. The study investigated the plasmid content and the diversity of plasmid-associated genes in a group of 152 hospital isolates of E. faecalis. The majority of plasmids visualized by pulsed-field gel electrophoresis of S1 nuclease-digested DNA fell into the range of 50–100 kb. PCR-based screening allowed detection of genes of the rep1pIP501, rep2pRE25, rep4pMBB1, rep6pS86, rep7pT181, rep8pAM373, rep9pAD1/pTEF2/pCF10, rep10pIM13 and rep13pC194 families in 29 different combinations. The par and ω-ε-ζ plasmid stabilization systems were ubiquitous (45 isolates, 29.6% and 88 isolates, 57.9%, respectively), while the axe–txe system was not found. The asa1 gene homologues encoding aggregation substance characteristic for the pAD1 and related group of pheromone-responsive plasmids were present in 106 isolates. A variety of sequence variants, including novel ones, of genes associated with pheromone-responsive plasmids, such as rep8pAM373, rep9pAD1/pTEF2/pCF10, par, and asa1 were observed. In conclusion, there is a big and only partially characterized pool of diverse plasmids in clinical E. faecalis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 70, Issue 3, November 2013, Pages 329–342
نویسندگان
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