کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2824257 | 1161636 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of a rolling-circle replication plasmid pXY3 from Lactobacillus plantarum XY3 Characterization of a rolling-circle replication plasmid pXY3 from Lactobacillus plantarum XY3](/preview/png/2824257.png)
The complete nucleotide sequence of cryptic plasmid pXY3 isolated from Lactobacillus plantarum strain XY3 has been determined. It consisted of a 2968-bp circular molecule with a G + C content of 39%. Sequence analysis of pXY3 revealed three putative open reading frames (ORFs). Based on sequence similarity, the Rep protein shared 89% and 88% identity with Rep proteins of pLF24 and pWCFS102, respectively, which belonged to the rolling-circle replication (RCR) pMV158 family. A ssoT-like single-strand origin (sso) and a typical pMV158 family double-strand origin (dso) located upstream of the rep gene. Southern blot analysis indicated pXY3 replicate via a rolling-circle (RC) mechanism. Furthermore, the relative copy number of pXY3 was estimated to be about 97 copies per chromosome equivalent by real-time PCR.
Journal: Plasmid - Volume 64, Issue 1, July 2010, Pages 36–40