کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2824416 1161652 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a versatile shuttle vector for gene expression in Geobacillus spp.
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Development of a versatile shuttle vector for gene expression in Geobacillus spp.
چکیده انگلیسی

An improved, versatile shuttle vector has been created for the metabolic engineering of Geobacillus spp. As kanamycin is the most thermo-tolerant of commonly used antibiotics, the gene encoding a thermostable kanamycin nucleotidyltransferase, together with the origin of replication from the G. stearothermophilus plasmid pBST1 were cloned into the Escherichia coli cloning vector pUC18. The resulting vector, named pUCG18, replicated in both organisms and could be transformed with an efficiency of 1 × 104 transformants per μg of DNA in G. thermoglucosidasius and was stable up to 68 oC with antibiotic selection. It was used to demonstrate expression of the pyruvate decarboxylase (pdc) gene from Zymomonas palmae in G. thermoglucosidasius at 45 oC.Sequence analysis of the pBST1 derived origin of replication revealed homology with a family of theta replicons that have previously only been found in strains of Bacillus megaterium.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 60, Issue 1, July 2008, Pages 45–52
نویسندگان
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