کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2824448 1161655 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
IHF- and SeqA-binding sites, present in plasmid cloning vectors, may significantly influence activities of promoters
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
IHF- and SeqA-binding sites, present in plasmid cloning vectors, may significantly influence activities of promoters
چکیده انگلیسی

Escherichia coli Integration Host Factor (IHF) regulates transcription from some bacterial and phage promoters by affecting DNA topology. Here we demonstrate that IHF affects transcription from bacteriophage λ pR promoter and the ptac promoter located on plasmids that contain IHF-binding sites. The IHF consensus sites are abundant and they can bind the IHF protein as shown in in vitro studies. The SeqA protein has a role in the complex regulation of pR activity, together with other factors altering DNA topology. Down-regulation of the transcription from ptac in the absence of IHF, together with IHF- and SeqA-mediated effects on pR, suggest that DNA topology cannot be underestimated when assessing in vivo promoters’ activity. This may have a significant impact on experiments employing recombinant genes cloned in plasmids and on choosing appropriate plasmid vectors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 60, Issue 2, September 2008, Pages 125–130
نویسندگان
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