کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2827573 | 1162453 | 2010 | 6 صفحه PDF | دانلود رایگان |
Heterozygous mutations in the ribosomal protein S19 (RPS19) gene are associated with Diamond-Blackfan anemia (DBA). The mechanism by which RPS19 mediates anemia are still unclear, as well as the regulation of RPS19 expression. We show herein that RPS19 binds specifically to the 5’ untranslated region of its own mRNA with an equilibrium binding constant (KD) of 4.1 ± 1.9 nM. We investigated the mRNA binding properties of two mutant RPS19 proteins (W52R and R62W) identified in DBA patients. We observed a significant increase in KD for both proteins (16.1 ± 2.1 and 14.5 ± 4.9 nM, respectively), indicating a reduced RNA binding capability (p < 0.05). We suggest that the binding of RPS19 to its mRNA has a regulatory function and hypothesize that the weaker RNA binding of mutant rRPS19 may have implications for the pathophysiological mechanisms in DBA.
Journal: Blood Cells, Molecules, and Diseases - Volume 45, Issue 1, 15 June 2010, Pages 23–28