کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2827795 | 1162466 | 2008 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reduced DIDS-sensitive chloride conductance in Ae1â/â mouse erythrocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
The resting membrane potential of the human erythrocyte is largely determined by a constitutive Clâ conductance â¼Â 100-fold greater than the resting cation conductance. The 4,4â²-diisothiocyanostilbene-2,2â²-disulfonic acid (DIDS)-sensitive electroneutral Clâ transport mediated by the human erythroid Clâ/HCO3â exchanger, AE1 (SLC4A1, band 3) is > 10,000-fold greater than can be accounted for by the Clâ conductance of the red cell. The molecular identities of conductive anion pathways across the red cell membrane remain poorly defined. We have examined red cell Clâ conductance in the Ae1â/â mouse as a genetic test of the hypothesis that Ae1 mediates DIDS-sensitive Clâ conductance in mouse red cells. We report here that wildtype mouse red cell membrane potential resembles that of human red cells in the predominance of its Clâ conductance. We show with four technical approaches that the DIDS-sensitive component of erythroid Clâ conductance is reduced or absent from Ae1â/â red cells. These results are consistent with the hypothesis that the Ae1 anion exchanger polypeptide can operate infrequently in a conductive mode. However, the fragile red cell membrane of the Ae1â/â mouse red cell exhibits reduced abundance or loss of multiple polypeptides. Thus, loss of one or more distinct, DIDS-sensitive anion channel polypeptide(s) from the Ae1â/â red cell membrane cannot be ruled out as an explanation for the reduced DIDS-sensitive anion conductance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Blood Cells, Molecules, and Diseases - Volume 41, Issue 1, JulyâAugust 2008, Pages 22-34
Journal: Blood Cells, Molecules, and Diseases - Volume 41, Issue 1, JulyâAugust 2008, Pages 22-34
نویسندگان
Seth L. Alper, David H. Vandorpe, Luanne L. Peters, Carlo Brugnara,