کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2827951 1162473 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of the effect of phenol and its derivatives on protein and free radical formation in human erythrocytes (in vitro)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Comparison of the effect of phenol and its derivatives on protein and free radical formation in human erythrocytes (in vitro)
چکیده انگلیسی

The effect of phenolic compounds: phenol, 2,4–dichlorophenol (2,4-DCP), 2,4-dimethylphenol (2,4-DMP) and catechol on human erythrocytes was studied. The level of fluorescent label – 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) oxidation by phenolic compounds in erythrocytes as well as the carbonyl group content and hemoglobin denaturation were monitored. H2DCFDA has been utilized extensively as a marker for studies of oxidative stress at the cellular level.We noted that 2,4-DCP, 2,4-DMP and catechol induced an increase in the concentration- and time-dependent H2DCFDA oxidation. We also observed an increase in carbonyl group content and the changes in parameter T (denaturation of hemoglobin) in erythrocytes incubated with 2,4-DCP, catechol and 2,4-DMP. The highest level of H2DCFDA oxidation was provoked by 2,4-DCP. The biggest changes of proteins in erythrocytes measured as the carbonyl group content were induced by 2,4-DMP, but measured as parameter T they were induced by catechol. It was observed that phenol did not oxidize H2DCFDA up to the concentration of 2.5 mM after 3 h of incubation. Phenol did not affect the carbonyl group content but decreased parameter T (induced denaturation of hemoglobin).To sum up, the kind of the substituent in a phenolic ring determines the molecular mechanism of action of the individual compound and the capacity of reactive oxygen species generation and thus damages the specified structures in human erythrocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Blood Cells, Molecules, and Diseases - Volume 39, Issue 3, November–December 2007, Pages 238–244
نویسندگان
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