کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2828111 1162476 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genetic relationship of a Mexican Afromestizo population through the analysis of the 3′ haplotype of the β globin gene in βA chromosomes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Genetic relationship of a Mexican Afromestizo population through the analysis of the 3′ haplotype of the β globin gene in βA chromosomes
چکیده انگلیسی
We analyzed 112 βA chromosomes from the Costa Chica region, with the aim of determining the 3′ haplotype (3′Hp) in Afromestizo individuals and its relationship with the reported populations. Thirty polymorphic sites were identified by sequencing and two by restriction fragment length polymorphisms. Genetic variability, genetic distances and neutrality tests were performed with the computer program Arlequin 3.0. Three groups were constructed, which we named 3kb-Hp, 330bp-Hp and 2.67kb-Hp with 32, 15 and 17 polymorphic sites respectively. In 3kb-Hp, 34 different 3′ haplotypes (14 of them new) were found; the three most common were 7B1 (17.8%), 7A1 (17.0%) and 1C1 (14.3%). 330bp-Hp revealed 18 different allelic sequences; the most frequent were the 1 (AT9T5, 22.3%), 2 (AT8T5, 20.5%) and 3 (AT7T7, 20.5%). 2.67kb-Hp displayed 14 distinct haplotypes, with B1 (30.3%), A1 (28.6%) and C1 (21.4%) having the highest frequencies. The gene diversity of the Costa Chica population was only significantly different to Gambia. In the genetic distances, the p values were not significant for Vanuatu, Sumatra and Central African Republic. The neutrality tests showed that the patterns of diversity in the Costa Chica population deviate significantly from the expectations of the standard neutral model. This is the first work performed in Mexico in which the extended 3′Hp was analyzed in βA chromosomes. The study showed clearly the presence of African and Asian genes in the Costa Chica population.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Blood Cells, Molecules, and Diseases - Volume 39, Issue 2, September–October 2007, Pages 169-177
نویسندگان
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