کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2828272 1162482 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Extracellular terbium and divalent cation effects on the red blood cell Na pump and chrysoidine effects on the renal Na pump
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Extracellular terbium and divalent cation effects on the red blood cell Na pump and chrysoidine effects on the renal Na pump
چکیده انگلیسی

We examined the effect of extracellular terbium (Tb3+) and divalent metal cations (Ca2+, Sr2+, and Ba2+) on 86Rb+ influx into rabbit and human red blood cells. We found that Tb3+ at 15 and 25 μM was a non-competitive inhibitor of 86Rb+ influx suggesting that Tb3+ is not binding to the transport site. This result reduces the usefulness of Tb3+ as a potential probe for the Eout conformation (the conformation with the transport site facing extracellularly). Ba2+, Sr2+ and Ca2+, at concentrations > 50 mM, had minimal effects on Rb+ influx into red blood cells (1 mM Rb-out). This suggests that the outside transport site is very specific for monovalent cations over divalent cations, in contrast to the inside transport site. We also found that chrysoidine (4-phenylazo-m-phenylenediamine) competes with Na+ for ATPase activity and K+ for pNPPase activity suggesting it is binding to the Ein conformation. Chrysoidine and similar compounds may be useful as optical probes of the Ein conformation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Blood Cells, Molecules, and Diseases - Volume 39, Issue 1, July–August 2007, Pages 7–13
نویسندگان
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