کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2828563 1162735 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structures of MBOgg1 in complex with two abasic DNA ligands
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Crystal structures of MBOgg1 in complex with two abasic DNA ligands
چکیده انگلیسی

7,8-Dihydro-8-oxoguanine (8-oxoG) is one of the most common oxidative DNA lesions. 8-oxoguanine DNA glycosylases (Oggs) detect and excise 8-oxoG through a multiple-step process. To better understand the basis for estranged base recognition, we have solved the crystal structures of MBOgg1, the 8-oxoguanine DNA glycosylase of Thermoanaerobacter tengcongensis, in complex with DNA containing a tetrahydrofuranyl site (THF, a stable abasic site analog) paired with an estranged cytosine (MBOgg1/DNATHF:C) or thymine (MBOgg1/DNATHF:T). Different states of THF (extrahelical or intrahelical) are observed in the two complexes of the ASU of MBOgg1/DNATHF:C structure. Analyses of their different interaction modes reveal that variable contacts on the 5′ region flanking the THF abasic site are correlated with the states of the THF. Comparison of MBOgg1/DNATHF:T with MBOgg1/DNATHF:C indicates that the non-preferred estranged T may affect MBOgg1’s contacts with the 5′ flank of the lesion strand. Furthermore, we identified a region in MBOgg1 that is rich in positive charges and interacts with the 5′ region flanking the lesion. This region is conserved only in non-eukaryotic Oggs, and additional mutagenesis and biochemical assays reveal that it may contribute to the distinct estranged base specificities between eukaryotic and non-eukaryotic Oggs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 181, Issue 3, March 2013, Pages 252–263
نویسندگان
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