کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2828706 1162752 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A concerted polymerization-mesophase separation model for formation of trichocyte intermediate filaments and macrofibril templates. 1: Relating phase separation to structural development
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
A concerted polymerization-mesophase separation model for formation of trichocyte intermediate filaments and macrofibril templates. 1: Relating phase separation to structural development
چکیده انگلیسی
The concept that macrofibril templates, the fibrillar precursor to complete macrofibrils incorporating matrix proteins in trichokeratins, are formed by intracellular anisotropic phase separation of intermediate filaments (IFs), is here developed in detail. The factors affecting structural development, including IF length dispersion, and presence of other macromolecular solutes, are discussed in terms of the statistical thermodynamic models presented over 30 years ago by P.J. Flory and co-workers. The crucial role of pendant IF head groups in controlling IF separation and stabilizing the mesophase is emphasised. In particular, a concerted process of polymerization of unit-length IF precursors coupled with continuous transfer of longer IFs to the anisotropic phase is invoked. Observed structures in differentiated cell lines in mature fibres are rationalised in terms of different possible nematic or double-twist liquid crystalline precursor structures, with varying degrees of anisotropic phase coalescence. The occurrence of rarely observed macromolecular double-twist structures is made plausible by qualitative analysis of mesophase mechanics and reference to alternative structures in other macromolecular mesogens. The model is consistent with, and explains, certain well-known features of mature fibre structure, such as filament-matrix ratios in different cell lines. A few comments relating to the infill of the template by keratin intermediate filament associated proteins (IFAPs) are presented.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 173, Issue 2, February 2011, Pages 229-240
نویسندگان
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