کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2829292 1162800 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structure of a transcriptional regulator TM1030 from Thermotoga maritima solved by an unusual MAD experiment
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Crystal structure of a transcriptional regulator TM1030 from Thermotoga maritima solved by an unusual MAD experiment
چکیده انگلیسی

The crystal structure of a putative transcriptional regulator protein TM1030 from Thermotoga maritima, a hyperthermophilic bacterium, was determined by an unusual multi-wavelength anomalous dispersion method at 2.0 Å resolution, in which data from two different crystals and two different beamlines were used. The protein belongs to the tetracycline repressor TetR superfamily. The three-dimensional structure of TM1030 is similar to the structures of proteins that function as multidrug-binding transcriptional repressors, and contains a large solvent-exposed pocket similar to the drug-binding pockets present in those repressors. The asymmetric unit in the crystal structure contains a single protein chain and the twofold symmetry of the dimer is adopted by the crystal symmetry. The structure described in this paper is an apo- form of TM1030. Although it is known that the protein is significantly overexpressed during heat shock, its detailed function cannot be yet explained.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 159, Issue 3, September 2007, Pages 424–432
نویسندگان
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