کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2829348 1162803 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A conserved role of Caenorhabditis elegans CDC-48 in ER-associated protein degradation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
A conserved role of Caenorhabditis elegans CDC-48 in ER-associated protein degradation
چکیده انگلیسی

Protein degradation mediated by the ubiquitin/proteasome system is ∗essential for the elimination of misfolded proteins from the endoplasmic reticulum (ER) to adapt to ER stress. It has been reported that the AAA ATPase p97/VCP/CDC48 is required in this pathway for protein dislocation across the ER membrane and subsequent ubiquitin dependent degradation by the 26S proteasome in the cytosol. Throughout ER-associated protein degradation, p97 cooperates with a binary Ufd1/Npl4-complex. In Caenorhabditis elegans two homologs of p97, designated CDC-48.1 and CDC-48.2, exist. Our results indicate that both p97 homologs interact with UFD-1/NPL-4 in a similar CDC-48UFD-1/NPL-4 complex. RNAi mediated depletion of the corresponding genes induces ER stress resulting in hypersensitivity to conditions which induce increased levels of unfolded proteins in the ER lumen. Together, these data suggest an evolutionarily conserved retro-translocation machinery at the endoplasmic reticulum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 156, Issue 1, October 2006, Pages 41–49
نویسندگان
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