کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2829564 | 1162814 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mechanical properties of cardiac titin's N2B-region by single-molecule atomic force spectroscopy
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mechanical properties of cardiac titin's N2B-region by single-molecule atomic force spectroscopy Mechanical properties of cardiac titin's N2B-region by single-molecule atomic force spectroscopy](/preview/png/2829564.png)
چکیده انگلیسی
Titin is a giant protein responsible for passive-tension generation in muscle sarcomeres. Here, we used single-molecule AFM force spectroscopy to investigate the mechanical characteristics of a recombinant construct from the human cardiac-specific N2B-region, which harbors a 572-residue unique sequence flanked by two immunoglobulin (Ig) domains on either side. Force-extension curves of the N2B-construct revealed mean unfolding forces for the Ig-domains similar to those of a recombinant fragment from the distal Ig-region in titin (I91-98). The mean contour length of the N2B-unique sequence was 120Â nm, but there was a bimodal distribution centered at â¼95Â nm (major peak) and 180Â nm (minor peak). These values are lower than expected if the N2B-unique sequence were a permanently unfolded entropic spring, but are consistent with the â¼100Â nm maximum extension of that segment measured in isolated stretched cardiomyofibrils. A contour-length below 200Â nm would be reasonable, however, if the N2B-unique sequence were stabilized by a disulphide bridge, as suggested by several disulphide connectivity prediction algorithms. Since the N2B-unique sequence can be phosphorylated by protein kinase A (PKA), which lowers titin-based stiffness, we studied whether addition of PKA (+ATP) affects the mechanical properties of the N2B-construct, but found no changes. The softening effect of PKA on N2B-titin may require specific conditions/factors present inside the cardiomyocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 155, Issue 2, August 2006, Pages 263-272
Journal: Journal of Structural Biology - Volume 155, Issue 2, August 2006, Pages 263-272
نویسندگان
Mark C. Leake, Anika Grützner, Martina Krüger, Wolfgang A. Linke,