کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2829887 1570656 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of transcriptional regulation of tetracycline responsive genes in Brugia malayi
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Analysis of transcriptional regulation of tetracycline responsive genes in Brugia malayi
چکیده انگلیسی

The Wolbachia endosymbiont of the human filarial parasites is necessary for parasite reproduction, making it an attractive chemotherapeutic target. Previous studies have demonstrated that mRNA levels of several nuclearly encoded genes are altered as a result of exposure to antibiotics that eliminate the endosymbiont, suggesting that they may be involved in maintaining the parasite–endosymbiont relationship. Here, we tested the hypothesis that the increase in mRNA levels of certain nuclearly encoded genes of Brugia malayi in response to tetracycline treatment involved specific regulatory elements present in the promoters of these genes. The promoters of three such genes (BmRPL13, BmRPS4 and BmHSP70) were tested for tetracycline responsiveness utilizing a homologous transient transcription system. Reporter gene expression driven by all three promoters was up-regulated in transfected embryos exposed to tetracycline. Substitution mutagenesis was employed to map the cis-acting elements responsible for this response in the BmHSP70 promoter. Tetracycline responsiveness was found to be distinct from the cis-acting elements involved in regulating the stress response from the BmHSP70 promoter; rather, tetracycline responsiveness was mediated by a TATAA-box like element. This study represents the first demonstration of small molecule-mediated gene regulation of a native B. malayi promoter.

Promoters of genes whose mRNA levels are increased by tetracycline treatment of B. malayi parasites in vivo are up-regulated in the presence of tetracycline.Figure optionsDownload high-quality image (80 K)Download as PowerPoint slideHighlights
► Tetracycline regulation of mRNA levels in B. malayi occurs at the transcriptional level.
► Tetracycline regulation of the BmHSP70 promoter does not involve the stress response.
► A TATAA-box like element is necessary for tetracycline regulation of BmHSP70.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 180, Issue 2, December 2011, Pages 106–111
نویسندگان
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