کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2829952 1163332 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oligopeptidase B deficient mutants of Leishmania major
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Oligopeptidase B deficient mutants of Leishmania major
چکیده انگلیسی

Oligopeptidase B is a clan SC, family S9 serine peptidase found in gram positive bacteria, plants and trypanosomatids. Evidence suggests it is a virulence factor and thus therapeutic target in both Trypanosoma cruzi and T. brucei, but little is known about its function in Leishmania. In this study L. major OPB-deficient mutants (Δopb) were created. These grew normally as promastigotes, had a small deficiency in their ability to undergo differentiation to metacyclic promastigotes, were significantly less able to infect and survive within macrophages in vitro, but were virulent to mice. These data suggest that L. major OPB itself is not an important virulence factor, indicating functional differences between trypanosomes and Leishmania in their interaction with the mammalian host. The possibility that an OPB-like enzyme (designated OPB2) in L. major might compensate for the loss of OPB in Δopb was investigated via by mapping its sequence onto the 1.6 Å structure of L. major OPB. This suggested that the residues involved in the S1 and S2 subsites of OPB2 are identical to OPB and hence the substrate specificity would be similar. Consequently there may be redundancy between the two enzymes.

We describe the generation of Leishmania major OPB-deficient mutants and a characterisation of their phenotype.Figure optionsDownload high-quality image (209 K)Download as PowerPoint slideResearch highlights▶ Oligopeptidase B knockout mutants generated in Leishmania major. ▶ Phenotype analysis shows that OPB is not a virulence factor. ▶ An OPB-like enzyme, OPB2, might compensate for loss of OPB.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 175, Issue 1, January 2011, Pages 49–57
نویسندگان
, , , , ,