کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2830259 | 1163371 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Congenicity and genetic polymorphism in cloned lines derived from a single isolate of a rodent malaria parasite Congenicity and genetic polymorphism in cloned lines derived from a single isolate of a rodent malaria parasite](/preview/png/2830259.png)
Many of the most commonly studied lines of the rodent malaria parasite Plasmodium yoelii yoelii originated from a single parasite isolate designated 17X. Amongst these lines, however, are parasites that exhibit variation in genotype and phenotype (e.g. growth rate). We describe here the results of a comparative genetic analysis between cloned lines of 17X that differ in growth rate, using nucleotide sequences of specific genes and patterns of genome-wide amplified fragment length polymorphism (AFLP). Our findings indicate that the original stock of 17X comprises two unrelated genotypes. Genotype-1 is represented by parasites with a slow growth phenotype (e.g. 17X (NIMR)) and a fast growth phenotype (e.g. 17XYM). Within this genotype, there are also genomic differences manifest as a small number of AFLP bands that differentiate the fast- and slow-growing lines from each other. The other genotype, genotype-2, is represented only by parasites with a slow growth phenotype (e.g. 17XA).
Journal: Molecular and Biochemical Parasitology - Volume 157, Issue 2, February 2008, Pages 244–247