کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2830935 1570730 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural and immunologic characterization of bovine, horse, and rabbit serum albumins
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Structural and immunologic characterization of bovine, horse, and rabbit serum albumins
چکیده انگلیسی

Serum albumin (SA) is the most abundant plasma protein in mammals. SA is a multifunctional protein with extraordinary ligand binding capacity, making it a transporter molecule for a diverse range of metabolites, drugs, nutrients, metals and other molecules. Due to its ligand binding properties, albumins have wide clinical, pharmaceutical, and biochemical applications. Albumins are also allergenic, and exhibit a high degree of cross-reactivity due to significant sequence and structure similarity of SAs from different organisms. Here we present crystal structures of albumins from cattle (BSA), horse (ESA) and rabbit (RSA) sera. The structural data are correlated with the results of immunological studies of SAs. We also analyze the conservation or divergence of structures and sequences of SAs in the context of their potential allergenicity and cross-reactivity. In addition, we identified a previously uncharacterized ligand binding site in the structure of RSA, and calcium binding sites in the structure of BSA, which is the first serum albumin structure to contain metal ions.


► First crystal structures of animal serum albumins from cattle, horse and rabbit.
► Identification of calcium transport sites on bovine albumin.
► Identification of potential epitopes; sequence divergence in structural context.
► Effect of albumin conservation on potential cross-reactivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 52, Issues 3–4, October–December 2012, Pages 174–182
نویسندگان
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