کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2831099 1570726 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A functional variant at miR-24 binding site in B7-H2 alters susceptibility to gastric cancer in a Chinese Han population
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
A functional variant at miR-24 binding site in B7-H2 alters susceptibility to gastric cancer in a Chinese Han population
چکیده انگلیسی


• rs4819388 is significantly related to the occurrence of gastric cancer.
• rs4819388 is significantly related to the clinical features of gastric cancer.
• The expression of B7-H2 is suppressed by miR-24.
• The inhibitory role of miR-24 is impacted by rs4819388.

A number of single nucleotide polymorphisms (SNPs) within the 3′-UTR of genes have been proved to be contributed to the risk of cancers. Here, we determined an SNP rs4819388 in the 3′-UTR of B7-H2 gene in 183 gastric cancer patients and 348 healthy controls by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis results showed that the rs4819388 genotypes were significantly related to the occurrence of gastric cancer. Compared with the GG homozygotes, the GA heterozygotes were significantly more prevalent in the patients (OR = 1.60, 95%CI = 1.08–2.37). In addition, the A allelic frequencies were significantly higher than that of the G allele in the patients with lymphatic metastasis (p = 0.034) and/or advanced TNM stage (p = 0.032). Dual-luciferase reporter assays showed that miR-24 inhibited the expression of B7-H2 through binding with the B7-H2 3′-UTR, and this inhibitory role of miR-24 was impacted by rs4819388. Our findings suggest that the SNP rs4819388 in B7-H2 3′-UTR, through disrupting the regulatory role of miR-24 on B7-H2 expression, contributes to the occurrence of gastric cancer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 56, Issues 1–2, November 2013, Pages 98–103
نویسندگان
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