کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2831155 | 1163782 | 2013 | 8 صفحه PDF | دانلود رایگان |
Rabbits generate a diversified primary antibody repertoire by somatically mutating, in gut-associated lymphoid tissue (GALT), an initial repertoire that is limited by preferential rearrangement of the 3′-most IGVH gene segment. To determine when repertoire diversification begins in GALT, we performed in situ hybridization on neonatal rabbit appendix sections with an activation-induced cytidine deaminase (AID) riboprobe, because AID is required for the mutational processes that diversify the primary antibody repertoire. We first detected AID mRNA expression around 1 week of age, in the basal region of developing follicles. By PCR-amplifying V–D–J genes from AID mRNA+ B cells isolated by laser capture microdissection, we found evidence of somatic hypermutation, and one likely instance of somatic gene conversion. Our results suggest that V–(D)–J gene diversification begins during early postnatal appendix development, in B cells stimulated to enter a proliferative program by signals derived from select intestinal commensals.
► Diversification of the rabbit primary antibody repertoire begins around 1 week of age in gut-associated lymphoid tissue.
► Only select members of the intestinal microbiota provide signals sufficient to drive the proliferative phase of rabbit appendix follicle development.
► VDJ gene diversification occurs during a B cell proliferative program induced by select members of the intestinal microbiota.
Journal: Molecular Immunology - Volume 54, Issue 2, June 2013, Pages 140–147