Effect of the format of antibodies on their specificity

► Effect of the antibody format on their specificity to antigens was investigated. ► The reducing of format antibody from full-scale MAb to scFv resulted in developing of polyspecific properties. ► Reverse construction of full-size MAb from scFv base followed to specificity and affinity improvement. ► Specificity and affinity properties of antibodies were not determined by the primary source of constant antibody domain. ► ScFv within a phage particle in comparison with soluble scFv possess a greater specificity to the antigen.