کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2832083 1163824 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Characterization of intracellular HLA-DR, DM and DO profile in K562 and HL-60 leukemic cells
چکیده انگلیسی

Surface class-II antigen expression fires-up the antigen presentation process and development of immune response. The absence of surface HLA-DR is used in various systems to avoid immune recognition. Most leukemic cells use such mechanism to escape immune surveillance. Here, K562 and HL-60 leukemic cells were examined as to intracellular HLA-DR, -DM and -DO expression, if any. Immunofluorescence scored by UV-microscopy, flow cytometry or confocal microscope analysis detected intracellular pools of HLA-DR, -DO and to a lesser degree HLA-DM, whereas sub-cellular fractionation localized these molecules within endosomes. RT-PCR experiments revealed the presence of HLA-DRαβ, HLA-DMαβ and HLA-DOβ but not HLA-DOα transcripts. Despite the absence of the HLA-DOα chain, stable transfectants of K562 with a full length HLA-DOβ-EGFP construct showed that DOβ chain could be translocated to endosomes and form stable complexes with HLA-DR. Such complexes could be responsible for arresting HLA-DR molecules within endosomes, maintaining their surface class-II negative state.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 45, Issue 15, September 2008, Pages 3965–3973
نویسندگان
, , , , , ,