کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2832192 1570744 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CD59 but not DAF deficiency accelerates atherosclerosis in female ApoE knockout mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
CD59 but not DAF deficiency accelerates atherosclerosis in female ApoE knockout mice
چکیده انگلیسی

Although the complement system has been implicated in atherosclerosis, the influence of membrane-bound complement regulators in this process has not been well understood. We studied the role of two membrane complement regulators, decay-accelerating factor (DAF) and CD59, in a murine model of atherosclerosis. DAF−/− and CD59−/− mice were crossed with apolipoprotein E (ApoE)-deficient mice to generate DAF−/−ApoE−/− and CD59−/−ApoE−/− mice. Mice were fed a high fat diet (HFD) for 8 or 16 weeks. En face analysis showed that CD59 deficiency led to more extensive lesions in female ApoE−/− mice both at 8 weeks (2.07 ± 0.27% vs.1.34 ± 0.21%, P = 0.06) and 16 weeks (17.13 ± 1.14% vs. 9.72 ± 1.14%, P < 0.001). Similarly, lesions measured by aortic root sectioning were larger in female CD59−/−ApoE−/− mice than in controls at 8 weeks of HFD feeding (20.74 ± 1.33% vs. 13.12 ± 1.46%, P < 0.005). On the other hand, DAF deficiency did not significantly influence atherosclerosis in ApoE−/− mice. Immunohistochemistry revealed more abundant membrane attack complex (MAC) deposition and more collagen staining in the aortic roots of CD59−/−ApoE−/− mice. Unexpectedly, total plasma cholesterol levels in female CD59−/−ApoE−/− mice were found to be elevated compared with CD59+/+ApoE−/− mice. We conclude that CD59 but not DAF offered protection in atherosclerosis in the context of ApoE deficiency. The protective role of CD59 was gender-biased and most likely involved prevention of MAC-mediated vascular injury, with possible contribution from an undefined effect on plasma cholesterol homeostasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 46, Issues 8–9, May 2009, Pages 1702–1709
نویسندگان
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