کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2832502 1163838 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning, expression and bioactivity of duck BAFF
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Cloning, expression and bioactivity of duck BAFF
چکیده انگلیسی

B cell activating factor (BAFF) belonging to the TNF family is critical for B cell survival and maturation. In the present study, we identified a duck BAFF cDNA, named dBAFF, by RT-PCR and RACE strategies. The open reading frame (ORF) of this cDNA encodes a 288-amino acid protein containing a predicted transmembrane domain and a putative furin protease cleavage site like chicken BAFF (cBAFF), human BAFF (hBAFF) and mouse BAFF (mBAFF). The amino acid identity between biologically soluble dBAFF and cBAFF, hBAFF or mBAFF is 97, 78 and 71%, respectively. RT-PCR analysis showed the dBAFF gene is strongly expressed in the bursa of fabricius. Recombinant soluble dBAFF (dsBAFF) fused with NusA.tag was efficiently produced in Origami B (DE3) pLysS expression host strain. In vitro, purified dsBAFF was not only able to promote survival of bursa B cells, but also able to co-stimulate proliferation of mammalian B cells with anti-IgM. Furthermore, recombinant hsBAFF has a positive effect on duck bursa B cells survival. These findings indicate dBAFF plays an important role in survival and proliferation of duck B cells and because of its high conservation in the evolution, functional cross-reactivity exists between mammalian and duck BAFF.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 44, Issue 6, February 2007, Pages 1471–1476
نویسندگان
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