کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2832747 | 1163844 | 2008 | 13 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering Reduction of the in vivo allergenicity of Der p 2, the major house-dust mite allergen, by genetic engineering](/preview/png/2832747.png)
The major allergen of the house-dust mite Dermatophagoides pteronyssinus, Der p 2, is recognized by approximately 90% of mite-allergic patients.We have produced two recombinant fragments of Der p 2 comprising aa 1–53 and aa 54–129 and a hybrid molecule (aa 54–129 + 1–53), combining the two fragments in inverse order, by genetic engineering. The recombinant Der p 2 derivatives were expressed in E. coli and purified to homogeneity. rDer p 2 derivatives (fragments and hybrid) showed a considerably reduced beta sheet structure and IgE reactivity compared to the Der p 2 wild-type allergen. The allergenic activity of the Der p 2 derivatives was reduced more than tenfold as evaluated in vitro in basophil activation assays and in vivo by skin prick testing of mite-allergic patients. Immunization of mice and rabbits with rDer p 2 derivatives induced Der p 2-specific IgG antibodies, which inhibited the binding of allergic patients’ IgE to Der p 2. Immunization of mice with rDer p 2 derivatives induced less allergenic IgE responses than immunization with rDer p 2. Thus the rDer p 2 derivatives exhibited less in vivo allergenic activity and allergenicity than the Der p 2 allergen but preserved immunogenicity and may hence represent candidates for specific immunotherapy of house-dust mite allergy.
Journal: Molecular Immunology - Volume 45, Issue 9, May 2008, Pages 2486–2498