کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2832861 1163849 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MicroRNA targets in immune genes and the Dicer/Argonaute and ARE machinery components
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
MicroRNA targets in immune genes and the Dicer/Argonaute and ARE machinery components
چکیده انگلیسی

We studied 613 genes which regulate immunity and, utilizing predictive algorithms, identified 285 genes as microRNA (miRNA or miR) targets. Of these, ∼250 are newly predicted gene–miR interactions. The frequency of predicted miRNA binding sites in immune gene 3′UTRs indicated preferential targeting of immune genes compared to the genome. Major targets include transcription factors, cofactors and chromatin modifiers whereas upstream factors, such as ligands and receptors (cytokines, chemokines and TLRs), were, in general, non-targets. About 10% of the immune genes were ‘hubs’ with eight or more different miRNAs predicted to target their 3′UTRs. Hubs were focused on certain key immune genes, such as BCL6, SMAD7, BLIMP1, NFAT5, EP300 and others. NF-κB and p53 do not themselves have binding sites for miRNAs but rather these pathways are targeted by miRNAs at downstream sites. MHC class II genes lacked miRNA targets but binding sites were identified in the CIITA gene and were shown experimentally to repress IFN-γ-induced MHC class II activation. Unexpectedly, factors involved in regulating message stability via AU-rich elements (ARE) were heavily targeted. Moreover, multiple components involved in the generation and effector functions of miRNAs (Dicer and Argonautes) were themselves miRNA targets suggesting that a subset of miRNAs may indirectly control their own production as well as other miRNAs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 45, Issue 7, April 2008, Pages 1995–2006
نویسندگان
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