کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2833442 | 1163872 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The activity of interleukin-4 receptor α-chain promoter is regulated by a GT box element The activity of interleukin-4 receptor α-chain promoter is regulated by a GT box element](/preview/png/2833442.png)
Interleukin-4 receptor (IL-4R) is the cell surface complex through which interleukin-4 (IL-4) signals exert its critical biological effects. The α-chain of IL-4R is responsible for the high affinity binding of IL-4. In this report, is characterized, the 5′ untranslated flanking region of murine IL-4Rα gene in the Th2 clone D10.G4.1. We have analyzed a DNA fragment spanning from −995 to +84 relative to the transcription start point. Mutagenesis analysis shows that, neither the previously described Stat6 (–395) nor the NFAT (−266) and NFκB (+25) sequences localized here, are involved in the IL-4Rα promoter activity. Reporter assays demonstrate that maximum transcriptional activity is achieved by the −89 to +84 sequence and this activity is independent of a TATA-like box located at −25. We have identified a GT box located at −45 as the critical element for the IL-4Rα promoter activity. Experiments in SL2 cells, which lack endogenous Sp proteins, show that IL-4Rα minimal promoter is transactivated by proteins of Sp family.
Journal: Molecular Immunology - Volume 43, Issue 11, April 2006, Pages 1808–1816